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系統識別號 U0007-2807201118022800
論文名稱(中文) 咬肌注射肉毒桿菌神經毒素對幼鼠 咬肌注射肉毒桿菌神經毒素對幼鼠海馬迴體區中CA1和CA3區神經細胞之影響
論文名稱(英文) Evaluation of hippocampal CA1 and CA3 neurons following Botox injection into masseter muscles in developing rats.
校院名稱 臺北醫學大學
系所名稱(中) 牙醫學系碩博士班
系所名稱(英) School of Dentistry
學年度 99
學期 2
出版年 100
研究生(中文) 王雅尼
研究生(英文) Ya-Ni Wang
學號 M204097008
學位類別 碩士
語文別 中文
口試日期 2011-06-22
論文頁數 80頁
口試委員 指導教授-蔡吉陽
共同指導教授-楊良友
委員-蕭裕源
中文關鍵字 咀嚼肌.肉毒桿菌,記憶 
英文關鍵字 masseter muscle , Botulinum toxin A, memory 
學科別分類
中文摘要 對於幼鼠咬肌注射BXTA使其咀嚼神經刺激傳導減少,造成腦部海馬迴體中CA1及CA3區神經細胞數目降低,可能影響其學習及記憶能力。由上述咬肌的活動力結果可以推論對於腦部的認知及學習記憶是有很大的相關連性,且不受年紀的大小所限制。
英文摘要 Evaluation the effects of masseter muscle function following BTXA injection were used mostly for diminishing excessive muscle activity (bruxism decrease) .The purpose of this study is to evaluation of hippocampal CA1 and CA3 neurons following BTXA injection in developing rats on histology level.
論文目次 致謝………………………………………………………………………
中文摘要………………………………………………………………Ⅳ
英文摘要………………………………………………………………Ⅵ
目錄………………………………………………………………… Ⅸ

第一章 緒論…………………………………………………………1
第一節 研究動機……………………………………………………2
第二節 研究目的……………………………………………………4
第三節 研究假設……………………………………………………4
第二章 文獻查證…………………………………………………… 5
第一節 肉毒桿菌神經毒素作用之機轉。…………………… 6
第二節 海馬迴體的構造與功能。………………………………10
第三節 動物實驗中空間記憶及學習能力的評估。……………13
第四節 過去動物實驗中減少咀嚼功能的方式。………………16
第五節 咀嚼對人體及動物全身的影響。………………………18
第六節 由動物實驗看咀嚼肌對空間記憶和學習能力的影。……20
第七節 由動物實驗看肌肉訓練對空間記憶和學習能力。………22
第八節 口腔至海馬迴體中可能的訊號傳遞…………………•…24
第三章 研究材料與方法……………………………………………27
第一節 研究對象…………………………………………………28
第二節 研究材料…………………………………………………29
第三節 研究設計及步驟…………………………………………30
第四節 統計方式…………………………………………………35

第四章 分析結果……………………………………………………36
第五章 討論……………………………………………………… 42
第六章 結論…………………………………………………………53
第七章 參考資料……………………………………………………55
第八章 附表…………………………………………………………61
Table.1 實驗分組……………………………………………………62
Table.2 實驗週數---紀錄大鼠隻數…………………………………63
Table.3.實驗組大白鼠與對照組大白鼠實驗期間之體重變化………64
Table.4.實驗組大白鼠與對照組大白鼠咬肌重量之比較……………65
Table.5海馬迴體中CA1、CA3、DG 區神經細胞數目的差異…………66
Table.6.海馬迴體中CA1、CA3、DG 區神經細胞(μm2)面積大小差異67

第九章 附圖…………………………………………………………68
fig.1 肉毒桿菌神經毒素結構圖………………………………………69
fig.2 肉毒桿菌神經毒素作用機轉圖…………………………………70
fig.3 Botulinum Toxin type A……………………………………70
fig.4 咬肌藥物注射點………………………………………………71
fig.5 每隻大鼠之海馬迴CA1、CA3、DG區中分析其細胞密度………72
fig.6 實驗期間兩組大鼠存活的個數…………………………………72
fig.7 兩組間大鼠的體重差異…………………………………………73
fig.8 實驗期間兩組體重上升差距……………………………………73
fig.9 控制組與實驗組兩組咬肌平均重量相比較……………………74
fig.10控制組與實驗組兩組神經細胞數目比較………………………75
fig.11控制組與實驗組兩組神經細胞 (μm2)面積大小差異………76
fig.12 組織切片下A與B海馬迴體神經細胞型態相比較………77
fig.13 Thionine染色下,組織切片海馬迴區CA1神經細胞比較78
fig.14 Thionine染色下,組織切片海馬迴區CA3神經細胞比較79
fig.15 Thionine染色下,組織切片海馬迴DG區神經細胞比較 80
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