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系統識別號 U0007-2707201121571100
論文名稱(中文) 藁本成分及其抑制黑色素生成之研究
論文名稱(英文) Studies on Constituents and Melanogenesis-Inhibitory Effects of Ligusticum sinense
校院名稱 臺北醫學大學
系所名稱(中) 生藥學研究所
系所名稱(英) Graduate Institute of Pharmacognosy
學年度 99
學期 2
出版年 100
研究生(中文) 朱怡慈
研究生(英文) Yi-Tzu Chu
學號 M303098006
學位類別 碩士
語文別 中文
口試日期 2011-07-11
論文頁數 158頁
口試委員 委員-李水盛
委員-郭悅雄
委員-林雲蓮
指導教授-梁文俐
共同指導教授-李慶國
中文關鍵字     酪胺酸酶  B16-F10黑色素瘤細胞 
英文關鍵字 Ligusticum sinense  Tyrosinase  B16-F10 melanoma cells 
學科別分類
中文摘要 中藥「藁本」又稱為「中國的獨活草」,來源是繖形科的 Ligusticum sinense Oliv. 之根莖,其主要功用為散寒解表、袪風勝濕、止痛。而事實上,古籍《神農本草經》亦有記載:藁本具有「長肌膚,悅顏色」之效。本研究的目的是分離鑑定藁本的成分及評估其對於美白的功效。
活性的評估,是藉由體外測定酪胺酸酶的抑制率及 B16 小鼠黑色素瘤细胞的黑色素生成量。藁本甲醇萃取物具有抑制 B16-F10 細胞黑色素生成的效果 (IC50 ≈ 50 μg/mL),但是不影響酪胺酸酶的酵素活性。經由乙酸乙酯、正丁醇及水的分配萃取後,乙酸乙酯層 (IC50 < 25 μg/mL) 進行開放矽膠管柱層析及高效能液相層析。
藉由活性追蹤的方式進行分離純化,總共鑑定出24個化合物,包含:8個 phenylpropanoids (GB1-8)、6個 phenolics (GB9-14)、2個 phthalide derivatives (GB15-16)、2個 polyacetylenic alcohols (GB17-18)、3個 terpenoids (GB19-21)、1個 steroid (GB22)、1個 fatty acid (GB23)、1個其他類 (GB24)。其中,GB5* 與 GB24* 為新化合物。化合物的結構是利用光譜分析與比對文獻確認。
結果顯示,藁本具有抑制黑色素生成之效。分離到的化合物中,以 GB16 有相對較佳的活性,在沒有細胞毒殺活性的情況下,可抑制 B16-F10 細胞黑色素生成 (IC50 = 31.1 +- 6.8 μM)。
英文摘要 The rhizoma of Ligusticum sinense Oliv. (Umbelliferae) is the traditional Chinese medicine “Gaoben”, as known as Chinese lovage, mainly used to expel wind-cold and to relieve pain. In fact, Shen Nong’s Herbal, the classic literature of TCM, also noted that Gaoben has a capability to light the skin color. The aims of this study were to isolate the chemical constituents and to evaluate the depigmenting effects of Gaoben.
Anti-melanogenesis properties were determined in vitro by the inhibition of mushroom tyrosinase and the produced melanin content in cultured B16 murine melanoma cells. The methanolic extract of Gaoben showed an inhibitory activity aganist melanogenesis in B16-F10 cells (IC50 ≈ 50 μg/mL), but no effect on tyrosinase activity. Via partitioning with EtOAC, n-Butanol, and H2O, the EtOAC layer (IC50 < 25 μg/mL) was subjected to silica gel open column and HPLC.
Bioassay-guided separation led to the identification of 24 compounds, including eight phenylpropanoids (GB1-8), six phenolics (GB9-14), two phthalide derivatives (GB15-16), two polyacetylenic alcohols (GB17-18), three terpenoids (GB19-21), one steroid (GB22), one fatty acid (GB23) and one other compound (GB24). Among them, GB5* and GB24* are new compounds. Those structures were identified by spectroscopic methods and comparisons with the published data.
The results indiacated that Gaoben showed the definite inhibition of melanin biosynthesis. Among these isolated constituents, GB16 was better than others. It had melanogenesis-inhibitory effect without direct cytotoxicity (IC50 = 31.1 +- 6.8 μM) in B16-F10 cells.
論文目次 標題頁 i
考試委員審定書 ii
論文書目同意公開申請書 iii
保密同意書暨簽到表 iv
誌謝 (Acknowledgements) vi
摘要 ix
Abstract x
目錄 (Contents) xi
表目錄 (Table of Contents) xiv
圖目錄 (Figure of Contents) xv
縮寫表 (Abbreviations) xix
第一章 緒論 (Introduction) 1
第一節 研究動機與背景 1
第二節 活性試驗論述 2
1-2.1 皮膚之介紹 2
1-2.2 皮膚之顏色 4
1-2.3 黑色素細胞 12
1-2.4 黑色素 14
1-2.5 黑色素生成及影響因素 15
1-2.6 美白之機轉 18
第三節 植物論述 23
1-3.1 本草文獻考察 23
1-3.2 藥用植物學考察 31
1-3.3 生藥學考察 34
1-3.4 藥理學及臨床應用考察 36
第四節 文獻回顧 37
第五節 研究目的 39
第二章 研究方法與材料 (Materials and Methods) 40
第一節 活性實驗 40
2-1.1 實驗藥品試劑 40
2-1.2 實驗設備 42
2-1.3 實驗方法 43
第二節 萃取與分離純化 47
2-2.1 分析儀器 47
2-2.2 溶媒試劑 49
2-2.3 實驗方法 50
第三章 結果 (Results) 59
第一節 植物分離之成分 59
第二節 化合物之結構解析 64
3-2.1 化合物GB4之結構解析 64
3-2.2 化合物GB5*之結構解析 70
3-2.3 化合物GB6之結構解析 78
3-2.4 化合物GB7之結構解析 84
3-2.5 化合物GB8之結構解析 88
3-2.6 化合物GB15之結構解析 92
3-2.7 化合物GB16之結構解析 97
3-2.8 化合物GB17之結構解析 103
3-2.9 化合物GB18之結構解析 110
3-2.10 化合物GB24*之結構解析 116
第三節 化合物之物理及光譜數據整理 122
第四節 活性實驗結果 135
3-4.1 粗萃物活性篩選 135
3-4.2 化合物活性評估 138
第四章 討論 (Discussion) 143
第五章 結論 (Conclusion) 145
參考文獻 (References) 146
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