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系統識別號 U0007-2607201023200800
論文名稱(中文) 苦瓜果實中苦瓜苷成分及其生物活性之探討
論文名稱(英文) Studies on Kuguaosides and their Bioactivities from the Momordica charantia Fruit
校院名稱 臺北醫學大學
系所名稱(中) 生藥學研究所
系所名稱(英) Graduate Institute of Pharmacognosy
學年度 98
學期 2
出版年 99
研究生(中文) 蕭萍君
研究生(英文) Ping-Chun Hsiao
學號 M303097014
學位類別 碩士
語文別 中文
口試日期 2010-06-21
論文頁數 220頁
口試委員 指導教授-徐鳳麟
共同指導教授-郭曜豪
委員-李水盛
委員-郭悅雄
委員-黃偉展
中文關鍵字 苦瓜;葫蘆烷型三萜  類;細胞毒殺;葡萄糖攝取;配糖體;葫蘆科 
英文關鍵字 Momordica charantia;glucose uptake;cytotxicity;cucurbitane-type triterpene;glycoside;Cucurbitaceae 
學科別分類
中文摘要 苦瓜 (Momordica charantia L.) 為一年生蔓性攀緣草本植物,為葫蘆科( Cucurbitaceae ),苦瓜屬 ( Momordica ) 的植物。苦瓜常被作為食用與藥用的植物,之前研究已證實苦瓜具有降血糖、抗腫瘤、抗發炎等功效。本研究以苦瓜果實70 % 酒精的萃取物,針對皂素活性成分並配合生物活性導向篩選 ( Bioactive-guided fractionation ) 進行分離與純化,總共分離得到二十九個葫蘆烷型 ( Cucurbitane-type ) 三萜類的化合物,包括十五個新化合物,分別為kuguaoside A (A1)、kuguaoside B (A2)、kuguaoside C (A3)、kuguaoside D (A4)、kuguaoside E (A5) 和kuguaoside F (B1)、kuguaoside G (B2)、kuguaoside H (B3)、kuguaoside I (B4)、kuguaoside J (B5)、kuguaoside K (B6)、kuguaoside L (B7)、kuguaoside M (B8)、kuguaoside N (B9)、kuguaoside O (B10),以及十四個已知化合物,momordicoside I (A6)、momordicoside F1 (A7)、momordicoside F2 (A8)、momordicoside K (A9)、momordicoside L (A10)、momordicoside U (A11)、goyaglycoside-b (A12)、goyaglycoside-d (A13)、7β,25-dihydroxycucurbita-5,23(E)-dien-19-al 3-O-β-D-
allopyranoside (A14) 、25-hydroxy-5β,19-epoxycucurbita-
6,23-dien-19-on-3β-ol 3-O-β-D-glucopyranoside (A15)、momordicine IV (B11)、goyaglycoside-a (B12)、kuguaglycoside C (B13)、3β,7β,25-trihydroxycucurbita-5,23(E)-dien-19-al (B14)。以上化合物皆藉由一維、二維核磁共振光譜等物理數據及化學方法鑑定化合物之結構。
藥理活性方面,在細胞毒殺 ( Cytotxicity ) 試驗中顯示,化合物A1、A2、A6、A7、A8、A9、A11、A12、A13、B1、B3、B4、B5、B6、B8、B12、B13對於人類乳癌細胞 ( MCF-7, Human breast adenocarcinoma )、人類結腸癌細胞 ( WiDr, Human colon adenocarcinoma ) 、人類喉癌細胞 ( HEp-2, Human laryngeal carcinoma ) 以及人類髓母細胞瘤 ( Doay, Human medulloblastoma ) 具有抑制癌細胞增生作用 ( IC50值介於 5~40 μg/ml之間),在葡萄糖攝取量 ( glucose uptake ) 測定中顯示,化合物A9、A14、B4、B5、B6、B7、B11有刺激C2Cl2肌肉母細胞 ( myoblast cell ),進而促進葡萄糖吸收的作用。結果顯示,苦瓜中葫蘆烷型的三萜類成分,可用以發展治療糖尿病或癌症藥物的潛力。
英文摘要 Momordica charantia L. (Cucurbitaceae), an annual vine, is widely distributed in tropical areas and is cultivated as vegetable crop and folk medicine in Taiwan. Previous investigations have shown that crude extracts of M. charantia possess antidiabetic (hypoglycemic), anti-tumor, anti-inflammatory activities. By column chromatography and bioassay-directed fractionation, twenty-nine cucurbitane-type triterpenoids including fifteen new compounds, kuguaoside A (A1), kuguaoside B (A2), kuguaoside C (A3), kuguaoside D (A4), kuguaoside E (A5), kuguaoside F (B1), kuguaoside G (B2), kuguaoside H (B3), kuguaoside I (B4), kuguaoside J (B5), kuguaoside K (B6), kuguaoside L (B7), kuguaoside M (B8), kuguaoside N (B9) and kuguaoside O (B10), along with fourteen known triterpene glycosides, momordicoside I (A6), momordicoside F1 (A7), momordicoside F2 (A8), momordicoside K (A9), momordicoside L (A10), momordicoside U (A11), goyaglycoside-b (A12), goyaglycoside-d (A13), and 7Momordica charantia L. (Cucurbitaceae), an annual vine, is widely distributed in tropical areas and is cultivated as vegetable crop and folk medicine in Taiwan. Previous investigations have shown that crude extracts of M. charantia possess antidiabetic (hypoglycemic), anti-tumor, anti-inflammatory activities. By column chromatography and bioassay-directed fractionation, twenty-nine cucurbitane-type triterpenoids including fifteen new compounds, kuguaoside A (A1), kuguaoside B (A2), kuguaoside C (A3), kuguaoside D (A4), kuguaoside E (A5), kuguaoside F (B1), kuguaoside G (B2), kuguaoside H (B3), kuguaoside I (B4), kuguaoside J (B5), kuguaoside K (B6), kuguaoside L (B7), kuguaoside M (B8), kuguaoside N (B9) and kuguaoside O (B10), along with fourteen known triterpene glycosides, momordicoside I (A6), momordicoside F1 (A7), momordicoside F2 (A8), momordicoside K (A9), momordicoside L (A10), momordicoside U (A11), goyaglycoside-b (A12), goyaglycoside-d (A13), and 7??,25-dihydroxycucurbita-5,23
(E)-dien-19-al 3-O-??-D-allopyranoside (A14), 25-hydroxy-????,19-epoxycucurbita-6,23
-dien-19-on-????-ol 3-O-??-D-glucopyranoside (A15), momordicine IV (B11), goyaglycoside-a (B12), kuguaglycoside C (B13), 3??,????,25-trihydroxycucurbita-5,23
(E)-dien-19-al (B14) were isolated and characterized glycosides constituents from
70 % EtOH extracts of the fruits of M. charantia.
The structural elucidation of these fifteen new and fourteen known compounds were based on spectroscopic analyses (mainly 1D and 2D NMR techniques), as well as chemical evidences.
Biological assay of cytotxicity showed that the A1, A2, A6, A7, A8, A9, A11, A12, A13, B1, B3, B4, B5, B6, B8, B12,and B13 possess moderate to promising cytotoxicity against several human tumor cell lines (MCF-7, WiDr, HEp-2, Doay, IC50 = 5~40 μg/ml). And in the glucose uptake test, A9, A14, B4, B5, B6, B7,and B11 could enhance the glucose uptake in C2Cl2 myoblast cell. These findings indicate that cucurbitane triterpenoids, the characteristic constituents of M. charantia, may provide leads as a class of therapeutic drugs against diabetes or cancer.
,25-dihydroxycucurbita-5,23
(E)-dien-19-al 3-O-β-D-allopyranoside (A14), 25-hydroxy-????,19-epoxycucurbita-6,23
-dien-19-on-3β-ol 3-O-β-D-glucopyranoside (A15), momordicine IV (B11), goyaglycoside-a (B12), kuguaglycoside C (B13), 3β,7β,25-trihydroxycucurbita-5,23
(E)-dien-19-al (B14) were isolated and characterized glycosides constituents from
70 % EtOH extracts of the fruits of M. charantia.
The structural elucidation of these fifteen new and fourteen known compounds were based on spectroscopic analyses (mainly 1D and 2D NMR techniques), as well as chemical evidences.
Biological assay of cytotxicity showed that the A1, A2, A6, A7, A8, A9, A11, A12, A13, B1, B3, B4, B5, B6, B8, B12,and B13 possess moderate to promising cytotoxicity against several human tumor cell lines (MCF-7, WiDr, HEp-2, Doay, IC50 = 5~40 μg/ml). And in the glucose uptake test, A9, A14, B4, B5, B6, B7,and B11 could enhance the glucose uptake in C2Cl2 myoblast cell. These findings indicate that cucurbitane triterpenoids, the characteristic constituents of M. charantia, may provide leads as a class of therapeutic drugs against diabetes or cancer.
論文目次 總目錄
中文摘要 I
英文摘要 II
總目錄 IV
圖目錄 V
表目錄 X
縮寫表 XII

第一章 緒論 1
第一節 研究背景與目的 1
第二節 苦瓜之植物型態 3
第三節 藥理與化學成分研究回顧 6
第二章 材料與方法 31
第一節 實驗儀器與藥品 31
第二節 實驗架構 33
第三節 苦瓜萃取與分離 34
第三章 化合物之結構證明 40
第一節 kuguaoside A (A1) 之結構解析 41
第二節 kuguaoside B (A2) 之結構解析 51
第三節 kuguaoside C (A3) 之結構解析 61
第四節 kuguaoside D (A4) 之結構解析 71
第五節 kuguaoside E (A5) 之結構解析 81
第六節 kuguaoside F (B1) 之結構解析 91
第七節 kuguaoside G (B2) 之結構解析 101
第八節 kuguaoside H (B3) 之結構解析 111
第九節 kuguaoside I (B4) 之結構解析 121
第十節 kuguaoside J (B5) 之結構解析 131
第十一節 kuguaoside K (B6) 之結構解析 141
第十二節 kuguaoside L (B7) 之結構解析 151
第十三節 kuguaoside M (B8) 之結構解析 161
第十四節 kuguaoside N (B9) 之結構解析 171
第十五節 kuguaoside O (B10) 之結構解析 181
第四章 生物活性試驗 191
第一節 細胞毒殺活性試驗 191
第二節 葡萄糖攝取量試驗 199
第五章 結論 204
第六章 參考文獻 209

參考文獻 1. Lebovitz, H. E.; Banerji, M. A., Treatment of insulin resistance in diabetes mellitus. Eur J Pharmacol 2004, 490, 135-146.
2. Musi, N.; Goodyear, L. J., Insulin resistance and improvements in signal transduction. Endocrine 2006, 29, 73-80.
3. Nahas, R.; Moher, M., Complementary and alternative medicine for the treatment of type 2 diabetes. Can Fam Physician 2009, 55, 591-596.
4. Carney, J. R.; Krenisky, J. M.; Williamson, R. T.; Luo, J., Achyrofuran, a new antihyperglycemic dibenzofuran from the South American medicinal plant Achyrocline satureioides. J Nat Prod 2002, 65, 203-205.
5. Nojima, H.; Kimura, I.; Chen, F. J.; Sugihara, Y.; Haruno, M.; Kato, A.; Asano, N., Antihyperglycemic effects of N-containing sugars from Xanthocercis zambesiaca, Morus bombycis, Aglaonema treubii, and Castanospermum australe in streptozotocin-diabetic mice. J Nat Prod 1998, 61, 397-400.
6. Kako, M.; Miura, T.; Nishiyama, Y.; Ichimaru, M.; Moriyasu, M.; Kato, A., Hypoglycemic activity of some triterpenoid glycosides. J Nat Prod 1997, 60, 604-605.
7. Oishi, Y.; Sakamoto, T.; Udagawa, H.; Taniguchi, H.; Kobayashi-Hattori, K.; Ozawa, Y.; Takita, T., Inhibition of increases in blood glucose and serum neutral fat by Momordica charantia saponin fraction. Biosci Biotechnol Biochem 2007, 71, 735-740.
8. Han, C.; Hui, Q.; Wang, Y., Hypoglycaemic activity of saponin fraction extracted from Momordica charantia in PEG/salt aqueous two-phase systems. Nat Prod Res 2008, 22, 1112-1119.
9. Grover, J. K.; Yadav, S. P., Pharmacological actions and potential uses of Momordica charantia: a review. J Ethnopharmacol 2004, 93, 123-132.
10. Grover, J. K.; Vats, V.; Rathi, S. S.; Dawar, R., Traditional Indian anti-diabetic
plants attenuate progression of renal damage in streptozotocin induced diabetic
mice. J Ethnopharmacol 2001, 76, 233-238.
11. Roffey, B. W.; Atwal, A. S.; Johns, T.; Kubow, S., Water extracts from Momordica
charantia increase glucose uptake and adiponectin secretion in 3T3-L1 adipose
cells. J Ethnopharmacol 2007, 112, 77-84.
12. Virdi, J.; Sivakami, S.; Shahani, S.; Suthar, A. C.; Banavalikar, M. M.; Biyani, M.
K., Antihyperglycemic effects of three extracts from Momordica charantia. J
Ethnopharmacol 2003, 88, 107-111.
13. Shih, C. C.; Lin, C. H.; Lin, W. L.; Wu, J. B., Momordica charantia extract on
insulin resistance and the skeletal muscle GLUT4 protein in fructose-fed rats. J
Ethnopharmacol 2009, 123, 82-90.
14. Francis, G.; Kerem, Z.; Makkar, H. P.; Becker, K., The biological action of saponins in animal systems: a review. Br J Nutr 2002, 88, 587-605.
15. Kren, V.; Martinkova, L., Glycosides in medicine: "The role of glycosidic residue in biological activity". Curr Med Chem 2001, 8, 1303-1328.
16. Chen, J. C.; Chiu, M. H.; Nie, R. L.; Cordell, G. A.; Qiu, S. X., Cucurbitacins and cucurbitane glycosides: structures and biological activities. Nat Prod Rep 2005, 22, 386-399.
17. Leung, L.; Birtwhistle, R.; Kotecha, J.; Hannah, S.; Cuthbertson, S.,
Anti-diabeticand hypoglycaemic effects of Momordica charantia (bitter melon): a
mini review. Br J Nutr 2009, 102, 1703-1708.
18. Celia, G.; Cummings, E.; David, A.P.; Jaipaul, S., Benehicial effect and
mechanism of action of Momordica charantia in the treatment of diabetes
mellitus: a mini review. Int J Diabetes & Metabolism 2003, 11, 46-55.
19. Cheng, H. L.; Huang, H. K.; Chang, C. I.; Tsai, C. P.; Chou, C. H., A cell-based
screening identifies compounds from the stem of Momordica charantia that
overcome insulin resistance and activate AMP-activated protein kinase. J Agric
Food Chem 2008, 56, 6835-6843.
20. Ojewole, J. A.; Adewole, S. O.; Olayiwola, G., Hypoglycaemic and hypotensive
effects of Momordica charantia Linn (Cucurbitaceae) whole-plant aqueous extract
in rats. Cardiovasc J S Afr 2006, 17, 227-232.
21. Akihisa, T.; Higo, N.; Tokuda, H.; Ukiya, M.; Akazawa, H.; Tochigi, Y.; Kimura,
Y.; Suzuki, T.; Nishino, H., Cucurbitane-type triterpenoids from the fruits of
Momordica charantia and their cancer chemopreventive effects. J Nat Prod 2007,
70, 1233-1239.
22. Jilka, C.; Strifler, B.; Fortner, G. W.; Hays, E. F.; Takemoto, D. J., In vivo
antitumor activity of the bitter melon (Momordica charantia). Cancer Res 1983,
43, 5151-5155.
23. Ray, R. B.; Raychoudhuri, A.; Steele, R.; Nerurkar, P., Bitter melon (Momordica
charantia) extract inhibits breast cancer cell proliferation by modulating cell cycle
regulatory genes and promotes apoptosis. Cancer Res 2010, 70, 1925-1931.
24. Bourinbaiar, A. S.; Lee-Huang, S., Potentiation of anti-HIV activity of
anti-inflammatory drugs, dexamethasone and indomethacin, by MAP30, the
antiviral agent from bitter melon. Biochem Biophys Res Commun 1995, 208,
779-785.
25. Lii, C. K.; Chen, H. W.; Yun, W. T.; Liu, K. L., Suppressive effects of wild bitter
gourd (Momordica charantia Linn. var. abbreviata ser.) fruit extracts on
inflammatory responses in RAW264.7 macrophages. J Ethnopharmacol 2009, 122
, 227-233.
26. Murakami, T.; Emoto, A.; Matsuda, H.; Yoshikawa, M., Medicinal foodstuffs.
XXI. Structures of new cucurbitane-type triterpene glycosides, goyaglycosides-a,
-b, -c, -d, -e, -f, -g, and -h, and new oleanane-type triterpene saponins,
goyasaponins I, II, and III, from the fresh fruit of Japanese Momordica charantia
L. Chem Pharm Bull 2001, 49, 54-63.
27. Okabe, H.; Miyahara, Y.; Yamauchi, T., Studies on the constituents of Momordica
charantia L. I Isolation and Characterization of Momordicosises A and B,
Glycosides of a Pentahydroxy cucurbitane Triterpene. Chem Pharm Bull, 1980,
28, 2753-2762.
28. Okabe H, Miyahara Y, Yamauchi T, et al. Studies on theconstituents of Momordica
charantia L.Ⅲ.Isolation andcharacterization of new cucurbitacin glycosides of the
immaturefruits. (2). Structures of momordicodicosides G, F1, F2 and I. Chem.
Pharm. Bull. 1982, 30, 3977-3986.
29. Liu, Y.; Ali, Z.; Khan, I. A., Cucurbitane-type triterpene glycosides from the
fruits of Momordica charantia. Planta Med 2008, 74, 1291-1294.
30. Matsuda, H.; Nakamura, S.; Murakami, T.; Structures of new cucurbitane
-type triterpenes and glycosides, karavilagenins D and E, and karavilosides VI,
VII, VIII, IX, X, and XI, from the fruit of Momordica charantia. Heterocycles
2007, 71, 331-341.
31. 潘輝、趙余慶,苦瓜化學成分研究進展,中藥研究與信息,2005, 7, p24-26.
32. 鄭嚮軍, 徐斌,董英,苦瓜化學成分的研究進展, 時珍國醫國藥,2006, 12,
p2449-2451.
33. 譚新武, 盧汝梅, 韋建華, 苦瓜化學成分的研究進展, 新醫學學刊, 2008, 6,
878-879.
34. 李健,陳姝娟等,苦瓜皂苷生物活性的研究發展, 食品研究與開發,2008, 29,
p144-146.
35. Harinantenaina, L.; Tanaka, M.; Takaoka, S.; Oda, M.; Mogami, O.; Uchida, M.;
Asakawa, Y., Momordica charantia constituents and antidiabetic screening of the
isolated major compounds. Chem Pharm Bull 2006, 54, 1017-1021.
36. 苗明三, 孫豔紅, 紀賤宇等, 苦瓜皂苷對實驗動物糖尿病模型的影響, 中國
中藥雜誌, 2008, 33, p845-847.
37. 柴瑞華, 關健等, 苦瓜皂苷降血糖作用的研究, 中草藥, 2008, 39, p746-747.
38. 趙海雯, 苦瓜皂苷降血糖功能的研究概況, 農產品加工學刊, 2007, 9, p24-25.
39. Tongia, A.; Tongia, S. K.; Dave, M., Phytochemical determination and extraction
of Momordica charantia fruit and its hypoglycemic potentiation of oral
hypoglycemic drugs in diabetes mellitus (NIDDM). Indian J Physiol Pharmacol
2004, 48, 241-244.
40. Miura, T.; Itoh, C.; Iwamoto, N.; Kato, M.; Kawai, M.; Park, S. R.; Suzuki, I.,
Hypoglycemic activity of the fruit of the Momordica charantia in type 2 diabetic
mice. J Nutr Sci Vitaminol 2001, 47, 340-344.
41. Ahmed, I.; Lakhani, M. S.; Gillett, M.; John, A.; Raza, H., Hypotriglyceridemic
and hypocholesterolemic effects of anti-diabetic Momordica charantia (karela)
fruit extract in streptozotocin-induced diabetic rats. Diabetes Res Clin Pract 2001,
51, 155-161.
42. Day, C.; Cartwright, T.; Provost, J.; Bailey, C. J., Hypoglycaemic effect of
Momordica charantia extracts. Planta Med 1990, 56, 426-429.
43. 明代. 李時珍, 本草綱目(二), 上海古籍出版社, 1991, p976
44. 趙中振,蕭培根, 當代藥用植物典(4), 香港賽馬會中藥研究院, p313-317.
45. Abascal, K.; Yarnell, E.; Using bitter melon to treat diabetes. Altern Complemen
Ther 2005, 11, 179-184.
46. Chen, J. C.; Chiu, M. H.; Nie, R. L.; Cordell, G. A.; Qiu, S. X., Cucurbitacins and
cucurbitane glycosides: structures and biological activities. Nat Prod Rep 2005, 22
, 386-399.
47. Chen, C.H. et al, Flora of Taiwan, second edition, Taiwan, 1993, 3, p 855,
860, 861 (台灣植物誌)
48. 全中和, 藥食兩用的東方蔬菜-苦瓜, 科學發展, 2007, 418, p10-13.
49. 黃祥益, 國產優良品牌苦瓜生產管理技術作業標準, 行政院農業
委員會高雄區農業改良場旗南分場
50. 全中和, 台灣野菜種源蒐集與利用之探討, 台灣植物資源之多樣性發展研討
會專刊, 2005, p141-149.
51. 邱年永、張光雄, 原色台灣藥用植物圖鑑 (2), 第一版, 台北南天書局發行,
1986, p235.
52. Ali, L.; Khan, A. K.; Mamun, M. I.; Mosihuzzaman, M.; Nahar, N.; Nur-e-Alam,
M.; Rokeya, B., Studies on hypoglycemic effects of fruit pulp, seed, and whole
plant of Momordica charantia on normal and diabetic model rats. Planta Med
1993, 59, 408-412.
53. Cakici, I.; Hurmoglu, C.; Tunctan, B.; Abacioglu, N.; Kanzik, I.; Sener, B.,
Hypoglycaemic effect of Momordica charantia extracts in normoglycaemic or
cyproheptadine-induced hyperglycaemic mice. J Ethnopharmacol 1994, 44,
117-121.
54. Kar, A.; Choudhary, B. K.; Bandyopadhyay, N.G., Comparative evaluation of
hypoglycaemic activity of some Indian medicinal plants in allocan diabetic rats.
J Ethnopharmacol. 2003, 84, 105-108.
55. Kumar, R.; Balaji, S.; Uma, T. S.; Sehgal, P. K., Fruit extracts of Momordica
charantia potentiate glucose uptake and up-regulate Glut-4, PPAR gamma and
PI3K. J Ethnopharmacol 2009, 126, 533-537.
56. 劉秀英, 苦瓜降血糖作用機制研究進展, 國外醫學(衛生學分冊), 2006, 33,
224-227.
57. Ahmed, I.; Adeghate, E.; Sharma, A. K.; Pallot, D. J.; Singh, J., Effects of
Momordica charantia fruit juice on islet morphology in the pancreas of the
streptozotocin-diabetic rat. Diabetes Res Clin Pract 1998, 40, 145-151.
58. Singh, A.; Singh, S. P.; Bamezai, R., Momordica charantia (Bitter Gourd) peel,
pulp,seed and whole fruit extract inhibits mouse skin papillomagenesis. Toxicol
Lett 1998, 94, 37-46.
59. 齊文波, 徐中平, 徐譽泰, 宋劍秋, 張英慧, 王海仁, 苦瓜素的分離純化與抗
腫瘤活性的研究, 離子交換與吸附, 1999, 15, 59-63.
60. 張瑜, 崔烔謨, 苦瓜腫瘤研究進展, 中國現代中藥, 2009, 11, 4-6.
61. 李春陽,賈文祥, 張雪梅, 馬巨輝, 張再容, 劉莉, 苦瓜蛋白誘發胃癌細胞
SGC7901 凋亡的研究, 四川腫瘤防治, 2001, 14, 1-4.
62. 鄭雪玲,苦瓜的降血糖功能, 科學發展, 2010, 446, 16-21.
63. Manabe, M.; Takenaka, R.; Nakasa, T.; Okinaka, O., Induction of
anti-inflammatory responses by dietary Momordica charantia L. (bitter gourd).
Biosci Biotechnol Biochem 2003, 67, 2512-2517.
64. Kobori, M.; Nakayama, H.; Fukushima, K.; Ohnishi-Kameyama, M.; Ono, H.;
Fukushima, T.; Akimoto, Y.; Masumoto, S.; Yukizaki, C.; Hoshi, Y.; Deguchi, T.;
Yoshida, M., Bitter gourd suppresses lipopolysaccharide-induced inflammatory
responses. J Agric Food Chem 2008, 56, 4004-4011.
65. Jiratchariyakul, W.; Wiwat, C.; Vongsakul, M.; Somanabandhu, A.; Leelamanit,
W.; Fujii, I.; Suwannaroj, N.; Ebizuka, Y., HIV inhibitor from Thai bitter gourd.
Planta Med 2001, 67, 350-353.
66. Lee-Huang, S.; Huang, P. L.; Chen, H. C.; Bourinbaiar, A.; Huang, H. I.; Kung, H.
F., Anti-HIV and anti-tumor activities of recombinant MAP30 from bitter melon.
Gene 1995, 161, 151-156.
67. 常鳳崗, 李建梅,苦瓜抗生育活性成分的化學研究(I), 中草藥, 1995, 26,
281-284.
68. Chan, W. Y.; Tam, P. P.; Yeung, H. W., The termination of early pregnancy in the
mouse by beta-momorcharin. Contraception 1984, 29, 91-100.
69. Chan, W. Y.; Tam, P. P.; So, K. C.; Yeung, H. W., The inhibitory effects of
beta-momorcharin on endometrial cells in the mouse. Contraception 1985, 31,
83-90.
70. Leung, S. O.; Yeung, H. W.; Leung, K. N., The immunosuppressive activities of
two abortifacient proteins isolated from the seeds of bitter melon (Momordica
charantia). Immunopharmacology 1987, 13, 159-171.
71. 王先遠, 金宏, 許志勤, 高蘭興, 苦瓜皂苷對老年荷瘤小鼠免疫功能的影響,
解放軍預防醫學雜誌, 2002, 20, 160-163.
72. Gurbuz, I.; Akyuz, C.; Yesilada, E.; Sener, B., Anti-ulcerogenic effect of
Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol
2000, 71, 77-82.
73. Braca, A.; Siciliano, T.; D'Arrigo, M.; Germano, M. P., Chemical composition and
antimicrobial activity of Momordica charantia seed essential oil. Fitoterapia
2008, 79, 123-125.
74. 傅明輝, 陳健漢, 莊冬紅, 苦瓜提取液的抗氧化、抑菌和降血糖活性, 食品科
學, 2001, 22, 88-90.
75. 吴志杰等, 苦瓜葉片抗蟲活性成分研究進展. 生物學通報, 2009, 44 , 12-14.
76. 凌冰, 王國才等, 苦瓜葉提取物對小菜蛾的拒食活性及有效成分研究. 中國
農業科學, 2008, 41, 3116-3122.
77. Wu, W.H.; Lin, B.Y.; Kuo, Y.H.; Huang, C.J,Triglycerides constituted of short and
medium chain fatty acids and dicarboxylic acids in Momordica charantia, as well
as capric acid, inhibit PGE2 production in RAW264.7 macrophages . Food Chem
2009, 117, 306-311.
78. Liu, C. H.; Yen, M.H.; Tsang, S.F.; Gan, K.H.; Hsu, H.Y., Antioxidant
triterpenoids from the stems of Momordica charantia. Food Chem. 2010, 118,
751-756.
79. Lago, J. H.; Brochini, C. B.; Roque, N. F., Terpenoids from Guarea guidonia.
Phytochemistry 2002, 60, 333-338.
80. Li, Q. Y.; Chen, H. B.; Liu, Z. M.; Wang, B.; Zhao, Y. Y., Cucurbitane
triterpenoids from Momordica charantia. Magn Reson Chem 2007, 45, 451-456.
81. Begum, S.; Ahmed, M.; Siddiqui, B.S., Triterpenes, a sterol and a monocyclic
alcohol from Momordica Charantia L. Phytochemistry 1997, 44, 1313-1320.
82. Miyahara, Y.; Okabe, H.; Yamauchi, T., Studies on the constituents of Momordica
Charantia L.Ⅱ. Isolation and characterization of minor seed glycosides,
momordicosides C, D and E. Chem Pharm Bull 1981, 29, 1561-1566.
83. 石雪萍, 姚惠源, 苦瓜皂苷的分離以及 PTP1B 抑制活性, 陝西師範大學學
報, 2008, 36, 63-67.
84. Nakamura, S.; Murakami, T.; Nakamura, J.; Kobayashi, H.; Matsuda, H.;
Yoshikawa, M., Structures of new cucurbitane-type triterpenes and glycosides,
karavilagenins and karavilosides, from the dried fruit of Momordica charantia L.
in Sri Lanka. Chem Pharm Bull 2006, 54, 1545-50.
85. Miyahara, Y.; Yamauchi, T.; Okabe, H., Studies on the constituents of Momordica
charantia L.Ⅳ.Characterization of the new cucurbitacin glycosides of the
immature fruits. (2). Structures of the bitter glycosides, momordicosides K and L .
Chem Pharm Bull 1982, 30, 4334-4340.
86. Chen, J.C.; Lua, L.; Zhanga, X. M.; Zhoua, Lin.; Lia, Z.R.; Qiu, M.H., Eight New
Cucurbitane Glycosides, Kuguaglycosides A–H, from the Root of Momordica
charantia L. Helv Chim Acta 2008, 91, 920-929.
87. Chuang, C. Y.; Hsu, C.; Chao, C. Y.; Wein, Y. S.; Kuo, Y. H.; Huang, C. J.,
Fractionation and identification of 9c, 11t, 13t-conjugated linolenic acid as an
activator of PPARγ in bitter gourd (Momordica charantia L.). J Biomed Sci
2006, 13, 763-72.
88. Tan, M. J.; Ye, J. M.; Turner, N.; Hohnen-Behrens, C.; Ke, C. Q.; Tang, C. P.;
Chen, T.; Weiss, H. C.; Gesing, E. R.; Rowland, A.; James, D. E.; Ye, Y.,
Antidiabetic activities of triterpenoids isolated from bitter melon associated with
activation of the AMPK pathway. Chem Biol 2008, 15, 263-273
89. Nguyen, X. N.; Phan, V. K.; Chau, V. M.; Ninh, K. B.; Nguyen, X. C.; Le, M. H.;
Bui, H. T.; Tran, H. Q.; Nguyen, H. T.; Kim, Y. H., Cucurbitane-type triterpene
glycosides from the fruits of Momordica charantia. Magn Reson Chem 2010, 48
, 392-396.
90. Chang, C.I.; Chen, C.R.; Liao, Y.W.; Cheng, H. L.; Chen, Y.C.; Chou, C.H.,
Cucurbitane-Type Triterpenoids from Momordica charantia. J. Nat. Prod. 2006,
69, 1168-1171.
91. Mekuria, D. B.; Kashiwagi, T.; Tebayashi, S.; Kim, C. S., Cucurbitane triterpenoid
oviposition deterrent from Momordica charantia to the leafminer, Liriomyza
trifolii. Biosci Biotechnol Biochem 2005, 69, 1706-1710.
92. Zhu, Z.J.; Zhong, Z.C.; Luo, Z.Y.; Xiao, Z.Y., Studies on the active constituents of
Momordica charantia L. Yao Hsueh Hsueh Pao 1990, 25, 898-903.
93. Yasuda, M.; Iwamoto, M.; Okabe, H., Structures of momordicine Ⅰ,Ⅱ and
Ⅲ.The bitter principles in the leaves and vines of Momordica Charantia L. Chem
Pharm Bull, 1984, 32, 2044-2047.
94. Yasui, H.; Kato, A.; Yazawa, M., Antifeedants to armyworms, Spodoptera litura
and Pseudaletia separate, form Bitter Gourd leaves, Momordica charantia. J. of
Chem. Ecol.. 1998, 24, 803-813.
95. Liu, J.Q.; Chen, J.C.; Wang, C.F.; Qiu, M.H., New Cucurbitane Triterpenoids and
Steroidal Glycoside from Momordica charantia. Molecules 2009, 14, 4804-4813.
96. Chen, J.; Tian, R.; Qiu, M.; Lu, L.; Zheng, Y.; Zheng, Z., Trinorcucurbitane and
cucurbitane triterpenoids from the roots of Momordica charantia. Phytochemistry
2008, 69, 1043-1048.
97. Chen, J.C.; Liu, W.Q.; Lu, L.; Qiu, M.H.; Zheng, Y.T.; Yang, L.M.; Zhang, X.
M.; Zhou, L.; Li, Z.R., Kuguacins F–S, cucurbitane triterpenoids from Momordica
charantia. Phytochemistry 2009, 70, 133-140.
98. Chang, C.I.; Chen, C.R.; Liao, Y.W.; Cheng, H. L.; Chen, Y.C.; Chou, C.H.,
Cucurbitane-Type Triterpenoids from the Stems of Momordica charantia. J Nat
Prod 2008, 71, 1327-1330.
99. Fatope, M.; Takeda, Y.; Yamashita, H., New cucurbitane triterpenoids from
Momordeca charantia L. J Nat Prod 1990, 53, 1491-1497.
100. Kuo, Y.H.; Huang, H.C.; Kuo Yang, L.M.; Lee, Y.M.; Lee, K.H.; Chang, F.R.;
Wu, Y.C., New dammarane-type saponins from the galls of Sapindus mukroossi,
J Agric Food Chem 2005, 53, 4722-4727.
101. Zang, D.Y.; Wu, J.; Ye, F.; Xue, L.; jiang, S.; Yi, J., Inhibition of cancer cell
proliferation and prostaglandin E2 synthesis by Scutellaria Baicalensis. Cancer
Res. 2003, 63, 4037-4043.
102. Baron, A.D.; Brechtel, G.; Wallace, P.; Edelman, S.V., Rates and tissue sites of
non-insulin-and insulin-mediated glucose uptake in human. Am J Physiol
1988, 255, 769-774.
103. Abe, F.; Mori, Y.; Yamauchi, T.; Saiki, Y., Strophanthidin Glycosides from
the roots of Apocynum venetum var. Basikurumon (Studies on Apocynum. II).
Chem Pharm Bull 1988, 36, 3811-3815.
104. Rhenius, M.; Porzel, A.; Diettrich, B.; Luckner, M., 21'-Di-dehydro-deacety
llanatoside C, a biotransformation product of deacetyllanatoside C from
senescent shoot cultures of Digitalis lanata. Phytochemistry 1997, 44,
1061-1064.
105. Yamauchi, T.; Abe, Fumiko.; Wan, S.C., Studies on Cerbera. IV. Polar
Cardenolide Glycosides from the Leaves of Cerbera adollam and Cerbera
manghas. Chem Pharm Bull 1987, 36, 4813-4818.
106. Chang, C.I.; Chen, C.R.; Liao, Y.W.; Cheng, H.L.; Chen, Y.C.; Chou, C.H.,
Cucurbitane-type triterpenoids from the stems of momordica charantia. J Nat
Prod 2008, 71, 1327-1330.
107. Ziel, F.H.; Venkatesan, N.; Davidson, M.B., Glucose transport is rate
limiting for skeletal muscle glucose metabolism in normal and STZ-induced
diabetic rats. Diabetes. 1988, 37, 885-890.
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