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系統識別號 U0007-2007201810525300
論文名稱(中文) 枸杞多醣體與藻藍素對阿斯匹靈誘發大鼠胃潰瘍修復作用之影響
論文名稱(英文) Effects of Lycium babarum polysaccharides and C -phycocyanin on healing of aspirin-induced gastric ulcer in rats
校院名稱 臺北醫學大學
系所名稱(中) 保健營養學研究所
系所名稱(英) Graduate Institute of Nutrition and Health Sciences
學年度 106
學期 2
出版年 107
研究生(中文) 謝書毓
研究生(英文) Shu-Yu Hsieh
學號 Ma07105017
學位類別 碩士
語文別 中文
口試日期 2018-07-02
論文頁數 90頁
口試委員 委員-蔡英傑
委員-楊素卿
指導教授-趙振瑞
中文關鍵字 胃潰瘍  阿斯匹靈  枸杞多醣體  藻藍素 
英文關鍵字 gastric ulcer  aspirin  Lycium barbarum polysaccharides  C-phycocyanin 
學科別分類
中文摘要 患有關節炎、心血管疾病會使用非類固醇抗發炎藥(nonsteroidal anti-inflammatory drugs, NSAIDs)治療,然而長期使用可能會導致胃潰瘍發生。先前研究指出,枸杞多醣體(Lycium barbarum polysaccharides, LBP)與藻藍素(C-phycocyanin, CPC)皆具有抗氧化、抗發炎等功能。然而尚未有研究探討給予胃潰瘍大鼠枸杞多醣體與藻藍素,是否能改善胃潰瘍與調節胃部微生物菌相。實驗將8週齡雄性Sprague-Dawley大鼠隨機分成五組(n=10-12):正常組、阿斯匹靈組、枸杞多醣體組(aspirin + 100 mg LBP/kg bw)、藻藍素組(aspirin + 50 mg CPC/kg bw)、枸杞多醣體+藻藍素組(aspirin + 50 mg LBP /kg bw + 25 mg CPC /kg bw)。於第一週至第九週給予LBP與CPC,第二週至第九週給予700 mg/kg bw阿斯匹靈誘發胃潰瘍。結果顯示,給予LBP能降低胃潰瘍分數與增加胃中雙岐桿菌屬,給予CPC能降低胃中脂質過氧化物及增加胃中雙岐桿菌屬;當合併給予LBP與CPC能降低胃中脂質過氧化物濃度、增加COX-1、NO、PGE2濃度及雙岐桿菌屬。對於阿斯匹靈誘發大鼠胃潰瘍,給予LBP能降低胃潰瘍指標,CPC可以改善氧化壓力,而合併給予LBP與CPC能增加胃部保護因子與改善氧化壓力,此外,補充LBP或CPC皆能增加雙岐桿菌屬,顯示無論LBP或CPC皆具有修復因阿斯匹靈誘發胃潰瘍的效果。
英文摘要 Non-steroidal anti-inflammatory drugs (NSAIDs) for the treatment of arthritis and cardiovascular disease are commonly associated with the occurrence of gastric ulcer. The objectives of this study were to investigate the effects of Lycium barbarum polysaccharides (LBP) and C-phycocyanin (CPC) on healing of aspirin-induced gastric ulcer in rats. Male Sprague-Dawley rats were divided into 5 groups: normal, aspirin, aspirin + LBP (100 mg/kg bw/d), aspirin+ CPC (50 mg/kg bw/d), and aspirin + LBP (50 mg/kg bw/d) + CPC (25 mg/kg bw/d). The treatments were given from the first week to the nineth week, and aspirin (700 mg /kg bw/d) was used to induce gastric ulcer in the second week to the nineth week. The treatment of LBP reduced ulcer score and CPC reduced MDA levels. The MIX group suppressed MDA levels, increased COX-1, NO, and PGE2 levels. Both treatments increased Bifidobacterium in the stomach. Therefore, after aspirin -induced gastric ulcer for 8 weeks in rats, LBP reduces ulcer index, and CPC inhibits oxidation in rats with aspirin-induced gastric ulcer. LBP combined with CPC improves gastroprotective factors and antioxidation. In addition, LBP or CPC could improve gastric microbiota. Both LBP and CPC have effects on healing of aspirin-induced gastric ulcer.
論文目次 中文摘要 I
AbstractII
致謝 III
目錄 V
圖目次 VIII
表目次 X
第一章 緒論 1
第二章 文獻回顧 3
第一節 胃功能與結構 3
第二節 胃潰瘍之成因與調節因子 5
第三節 使用NSAIDs的族群 8
第四節 阿斯匹靈誘發胃潰瘍之動物模式與機制 9
第五節 胃潰瘍的治療方式 13
第六節 枸杞多醣體簡介與相關研究 15
第七節 藻藍素簡介與相關研究 18
第八節 枸杞多醣體與藻藍素對胃潰瘍之影響 20
第九節 研究目的與動機 21
第三章 研究設計與材料方法 22
第一節 實驗設計 22
一、 實驗動物 22
二、 實驗設計 22
三、 動物飼料 25
四、 阿斯匹靈 25
五、 枸杞多醣體 25
六、 藻藍素 29
第二節 檢測項目及方法 31
一、胃潰瘍指標 31
二、胃部保護因子 32
三、胃部抗氧化指標 35
四、胃部發炎指標 36
五、胃部微生物菌相分析 39
六、總蛋白質定量方法 40
第三節 統計分析 42
第四章 結果 43
第一節 大鼠體重與攝食量 43
第二節 胃潰瘍指標 45
第三節 腺胃切片病理學檢查 48
第四節 胃部保護因子指標 52
第五節 胃抗氧化指標 57
第六節 胃發炎指標 59
第七節 胃部微生物菌相 64
第五章 討論 70
第一節 動物模式建立 70
第二節 動物體重、攝食量及阿斯匹靈攝取量 72
第三節 LBP與CPC對胃潰瘍指標之影響 73
第四節 LBP與CPC對胃部保護因子的影響 74
第五節 LBP與CPC對胃部抗氧化指標的影響 76
第六節 LBP與CPC對胃部發炎指標的影響 77
第七節 LBP與CPC對胃中微生物菌相的影響 78
第八節 綜合討論 80
第六章 結論 83
第七章 參考資料 84
附件一 實驗動物照護及使用委員會審查同意書 90

圖目次

圖 一、胃組織圖 4
圖 二、胃黏膜屏障 6
圖 三、胃潰瘍形成機制 7
圖 四、NSAIDs作用機制圖 12
圖 五、枸杞、枸杞多醣體粉末與其化學結構式 17
圖 六、螺旋藻、藻藍素粉末與其化學結構式 19
圖 七、實驗流程設計圖 24
圖 八、以HPAEC-PAD分析LBP單醣組成之層析圖譜27
圖 九、大鼠胃潰瘍的外觀 47
圖 十、以H&E染色之腺胃切片(100×) 50
圖 十一、以H&E染色之腺胃切片(40×) 51
圖 十二、LBP與CPC對胃中COX濃度的影響 54
圖 十三、LBP與CPC對胃中PGE2濃度的影響 55
圖 十四、LBP與CPC對胃中NO濃度的影響 56
圖 十五、LBP與CPC對胃中抗氧化指標的影響 58
圖 十六、LBP與CPC對胃中NF-κB (p65)的影響 61
圖 十七、LBP與CPC對胃中ICAM-1的影響 62
圖 十八、LBP與CPC對胃中發炎與抗發炎指標的影響 63
圖 十九、α-物種歧異度分析 66
圖 二十、LDA值分佈柱狀圖與LEfSe進化分支圖 68
圖 二十一、LBP與CPC對胃中比菲德氏菌相對豐富度的影響 69

表目次

表 一、 枸杞多糖體之單醣組成 . 28
表 二、 螺旋藻萃取物組成分 30
表 三、 大鼠體重、食物攝取量及阿斯匹靈攝食量44
表 四、 LBP與CPC對胃損傷的影響 46
表 五、 腺胃切片病理學評分 49
表 六、 LBP與CPC對胃中微生物菌門分布的影響 67
表 七、 不同介入對不同指標的影響 82


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